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Cytogenetics Services

 

We are not currently experiencing any issues receiving samples from Europe. When preparing a shipment, please include the following statements on the Customs Invoice: "The packed material is classified as 'not restricted'. The material will be used for laboratory research only. The material contains no pharmaceutical properties."

Our cytogenetics services are continuing to run as normal throughout lockdown. If you would like to request further information on these services, then please contact tech@cellgs.com

Genetic Stability

Cells grown in vitro, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), are prone to acquiring genetic abnormalities. Long term passaging and the genetic manipulation of cells can cause the development of such aberrations. This can undermine research and lead to misleading results in downstream experiments.

Methods of testing

Karyotyping detects important balanced translocations and as such remains the gold standard of genetic audit required by regulatory agencies and journals. Array analysis is also beneficial as it provides a greater degree of resolution and can identify microdeletions that may be missed by karyotype analysis. Combine both analyses to achieve a comprehensive examination of genomic integrity.

 

Karyotype vs Array 

 

Karyotype

Array

Mosaicism detection

individual cells note 1

>15-20% of cells

Resolution

30 Mb

less than 200 kb 
Copy number detection Yes

Yes

Balanced translocation detection Yes

No

Note 1 Statistical consideration required. e.g. 20 cell analysis will not always detect 5% mosaicism.