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Cytogenetics Services

 

Cell Guidance Systems is a leading provider of cytogenetics services. We provide:

  • G-banding karyotype analysis.  The gold standard for the identification of a range of balanced and unbalanced chromosomal abnormalities even in low-level mosaic cell populations
  • Array Comparative Genome Hybridization (aCGH) and Array Genome Hybridization (AGH). To identify unbalanced and  abnormalities at a higher resolution

Service offerings 

     

 

Karyotype vs Array 

 

Karyotype

Array

Mosaicism detection

individual cells note 1

>15-20% of cells

Resolution

30 Mb

less than 200 kb 
Copy number detection Yes

Yes

Balanced translocation detection Yes

No

 Note 1 Statistical consideration required. e.g. 20 cell analysis will not always detect 5% mosaicism.

 

Choosing a service for cytogenetic screening

Cells grown in vitro are prone to genetic instability and chromosomal loss. In particular, cells maintained over a long number of passages, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), are prone to acquiring genetic abnormalities. The manipulation of cells using CRISPR-Cas9 has also been associated with the development of genetic abnormalities which undermine the relevance of cells and may lead to misleading results in downstream experiments.

Only karyotyping detects important balanced translocations and as such remains the gold standard of genetic audit required by regulatory agencies and journals. Array analysis is also beneficial as it provides a greater degree of resolution and can identify microdeletions that may be missed by karyotype analysis. The depth of analysis should be proportional to downstream requirements. In general, most labs will routinely karyotype important cell lines. 

Cell Guidance Systems' cytogenetics services are carried by experienced, clinically qualified cytogeneticists.