Karyotype service for live cells

Code Description Price Qty
K02 Karyotype service from live human cells, BSL1 $695.00
K06 Karyotype service from live mouse cells, BSL1 $695.00
K07 Karyotype service from live human cells, BSL2 $770.00
K08 Karyotype service from live mouse cells, BSL2 $770.00

Service availability

This schedule is for reference only. Please contact us at info@cellgs.com to schedule sample arrival.

Protocols and workflow

Biosafety level

We are able to process both BSL1 and BSL2 samples. In order to accept and process live cells classed as BSL2, we require evidence of the below screening:

• Confirmation via PCR or equivalent test that the sample material is free of human pathogens (HBV, HCV, HIV-1, HIV-2, HTLV I/II, CMV, EBV, HSV-1, HSV-2) from a CLIA-certified lab.
• Confirmation via PCR or equivalent that the sample is free of mycoplasma.

Samples received without evidence of screening will be disposed of on arrival

Live cell requirements

Please strictly adhere to the following requirements for live samples:

  • Cells are shipped at room temperature
  • Shipment flasks are full to the brim with cell culture media (approximately 60 ml for a T25 flask)
  • Flasks are shipped with solid, leakproof caps attached
  • Additional flasks with filter caps are provided (1 per sample)
  • Enough additional cell culture media is supplied in order to allow for sufficient media changes (20 ml per flask)
  • Samples should be shipped to arrive at Cell Guidance Systems’ laboratories, two days prior to reaching 100% confluency. Samples should be in log phase at the time of fixing
  • Samples should be in transit for no more than 24 hours, and reach us on a Tuesday or Wednesday at the latest
  • Please do not send any samples without booking in advance

If you have any questions, please contact us by email at info@cellgs.com. We will respond within one business day.

Citations

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Schöndorf DC, Elschami M, Schieck M, Ercan-Herbst E, Weber C, Riesinger Y, Kalman S, Steinemann D, and Ehrnhoefer DE. Generation of an induced pluripotent stem cell cohort suitable to investigate sporadic Alzheimer's Disease. (2018). Stem Cell Research Jan;34:101351.

Joshi PS, Modur V, Cheng J, Robinson K and Rao K. Characterization of immortalized human mammary epithelial cell line HMEC 2.6. (2017). Tumour biology Oct;39(10):1010428317724283.

Tidball AM, Dang LT, Glenn TW, Kilbane EG, Klarr DJ, Margolis JL, Uhler MD, and Parent JM. Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing. (2017). Stem Cell Reports 9(3): 725-731.

Ludtmann MHR, Arber C, Bartolome F, de Vicente M, Preza E, Carro E, Houlden H, Gandhi S, Wray S, and Abramov AY. Mutations in valosin-containing protein (VCP) decrease ADP/ATP translocation across the mitochondrial membrane and impair energy metabolism in human neurons. (2017). Journal of Biological Chemistry 292(21): 8907-8917.

Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, and Freude KK. Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B. (2016). Stem Cell Research 17(1): 148-150.

Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, et al. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. (2015). Nature Communications 6: 7095.