Exo-spin™ columns

Code Description Price Qty
EX03-8 Exo-spin™, 8 columns $90.00
EX03-25 Exo-spin™, 24 columns $250.00
EX03-50 Exo-spin™, 48 columns $435.00
EX06-30 Exo-spin™ buffer, 30 ml $50.00
EX06-250 Exo-spin™ buffer, 250 ml $235.00
Exo-spin™ columns

Product description

Exo-spin™ products provide a proven, rapid and reliable way of generating high quality purified exosomes suitable for a range of research applications.

Exo-spin™ technology is based on size exclusion chromatography (SEC), which is reliable for exosome isolation. A concentration step is recommended if a starting sample has low exosome-content. This includes cell culture media, urine, saliva etc. Without this important step, a lower exosome recovery will be obtained. Exo-spin™ provides a simple protocol which allows you to purify your sample in less than 2 hours with consistent and reliable results.

The EX03 Exo-spin™ product contains pre-packed and equilibrated ready-to-use SEC columns. It can be used alone with sera and plasma or in combination with concentrating methods, such as precipitation, for other sample types.

The EX03 Exo-spin™ columns are the same as found in EX01 and EX02 kits and will process any sample smaller than 100 µl. In addition to purifying exosomes from biological samples, Exo-spin™ columns are also very effective for the removal of excess dye in fluorescently labelled exosome samples.

Products on this page contain Exo-spin™ mini columns

 

Which Exo-spin™ kit shall I choose for my sample volume?

 

Low exosome-content biofluids (up to 1x109/ml)

including cell culture media, saliva, cerebrospinal fluid, urine

Sample Volume

isolation method

Exo-spin Kit

column bed length

< 1ml to 50 ml 

concentration + SEC

EX01 mini

1.3 cm 

 < 1 ml to 75 ml Note 1

concentration + SEC

EX05 mini-HD

6.35 cm

75 ml to 500 ml Note 1 concentration + SEC EX04 midi 2.15 cm

 

Plasma and Sera

Sample Volume

isolation method

Exo-spin Kit

column bed length

< 100 µl Note 2 

SEC

EX03 mini columns

1.3 cm 

< 150 µl 

SEC

EX05  mini-HD 6.35 cm 
100 µl - 250 µl Note 3 concentration + SEC EX02 mini blood 1.3 cm  
1 ml SEC EX04 midi 2.15 cm 

Note 1:  For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides. 
Note 2: Sera only. Note 3: Up to 500 ul for sera.
 

Exo-spin™ column and resin pore size

The column bed volume in EX02 Exo-spin™ mini-columns is 500 µl, allowing for 100 µl of volume to be loaded onto each column.

The pore size within the resin is approximatively 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column. 

Highest recovery and purity

Exosomes in a range 30-300 nm will elute in the first fraction. All other proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for downstream application.

Reproducibility

All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification columns and Exo-spin™ Buffer at 4°C. All other components should be stored at room temperature (15°C - 25°C).

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

If you are just starting to work with exosomes, consider our starter pack

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. Complete details can be found in the product page here.

 

Product data

EX03 Exo-spin™ columns comparative data

NTARNA analysis

NTA measurement of samples prepared from cell culture medium: (a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including (b) ultracentrifugation (c) competitor kit. The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation. Lower rRNA contamination levels Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following (d) Exo-spin™ purification from cell culture samples compared with (e) ultracentrifugation or (f) competitor kit.
Exosomes extraction profile
Exosomes purified with Exo-spin™ columns primarily elute in the range between 100 μl and 300 μl. Collecting this 200 μl fraction considerably enriches for exosomes over proteins and smaller molecules. Starting sample is 100 μl mouse serum. 
 

Characterizing Exo-spin™ isolated exosomes using nanoparticle tracking analysis (NTA)

Nanoparticle Tracking Analysis NTA

Exosome characterization with NTA. Exosomes have been isolated using the Exo-spin™ kit and analysis performed with the ZetaView® instrument.

Downstream applications: advice and content

The EX03 Exo-spin™ kit is compatible with all downstream application and has been published in a large range of different applications.

Mass spectrometry:

  • Campos A, Salomon C, Bustos R, Díaz J, Martínez S, Silva V, Reyes C, Díaz-Valdivia N, Varas-Godoy M, Lobos-González L and Quest AF. (2018). Caveolin-1-containing extracellular vesicles transport adhesion proteins and promote malignancy in breast cancer cell lines. Nanomedicine 13(20):2597-2609.

Exosome labelling:

  • Shu SL, Yang Y, Allen CL, Maguire O, Minderman H, Sen A, Ciesielski MJ, Collins KA, Bush PJ, Singh P, Wang X, Morgan M, Qu J, Bankert RB, Whiteside TL, Wu Y and Ernstoff MS. (2018). Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Scientific Reports 8: 12905.

Citations

Campos A, Salomon C, Bustos R, Díaz J, Martínez S, Silva V, Reyes C, Díaz-Valdivia N, Varas-Godoy M, Lobos-González L and Quest AF. (2018). Caveolin-1-containing extracellular vesicles transport adhesion proteins and promote malignancy in breast cancer cell lines. Nanomedicine 13(20):2597-2609.

Shu SL, Yang Y, Allen CL, Maguire O, Minderman H, Sen A, Ciesielski MJ, Collins KA, Bush PJ, Singh P, Wang X, Morgan M, Qu J, Bankert RB, Whiteside TL, Wu Y and Ernstoff MS. (2018). Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Scientific Reports 8: 12905.

Piao YJ, Kim HS, Hwang EH, Woo J, Zhang M and Moon WK. (2017). Breast cancer cell-derived exosomes and macrophage polarization are associated with lymph node metastasis. Oncotarget 9(7): 7398–7410.

E L Kavanagh, S Lindsay, M Halasz, L C Gubbins, K Weiner-Gorzel, M H Z Guang, A McGoldrick, E Collins, M Henry, A Blanco-Fernández, P O' Gorman, P Fitzpatrick, M J Higgins, P Dowling, and A McCann. (2017). Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis 6(10): e388.

Koo KM, Wee EJ and Trau M.; (2016). Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via Recombinase Polymerase Amplification. Theranostics 6(9): 1415–1424.

Yoon C, Kim J, Park G, Kim S, Kim D, Hur DY, Kim B and Kim YS. (2016). Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt's lymphoma exosomes. Tumor Biology 37(1):313-21.