Exosomes are extracellular vesicles (EVs) released by cells, which are present in biological fluids and in cell culture conditioned media. Their quantification and characterization are important steps with general international rules (see MISEV 2018 guidelines) recommending measurements from at least two of the following three parameters: particle number, protein, or lipid amount.
Principles of nanoparticle tracking analysis (NTA)
NTA is a technique used for the simultaneous measurement of the size and concentration of single nanoparticles with high precision and reproducibility.
A laser directed at the sample solution generates light scattering by the particles in the sample. The NTA software captures the Brownian motion of each individual particle in a frame-by-frame video. Large and small particles have different diffusion movements in solution which allows for the calculation of the hydrodynamic diameter of each particle using the Stokes-Einstein equation.
Cell Guidance Systems service uses ZetaView®, a second-generation instrument from Particle Metrix GmbH, which performs rapid in vitro measurement of physical parameters, including size, surface charge, and concentration of particles.
The ZetaView® instrument has been designed for automated scanning NTA. To improve representative analysis, the software measures 11 distinct positions in each sample providing further information than single-point assessments, thus contributing to repeatable and reproducible results. Furthermore, automated parameters for auto-alignment and autofocus ensure that the video quality is kept constant while the particle movement is recorded.
This technology is well suited for use in exosome characterization and is compatible with low concentrated and polydisperse samples.
NTA size profiling service
Full video, raw data and complete report provided for each sample
Full reports emailed within 5 to 10 business days
Set up charge and sample price allow affordable analysis
Please contact us if you have any questions about this service.