A compelling method for isolating exosomes > SEC

A compelling method for isolating exosomes  > SEC

A compelling method for isolating exosomes: size exclusion chromatography (SEC)

Exo-spin™ SEC columns can be used as a stand-alone product or as part of a combined strategy for isolating small EVs!

CellGS offers pre-packed and equilibrated ready-to-use SEC columns!

With the pore size of the resin being approximately 30 nm, Exo-spin™ SEC columns offer a highly pure exosome elution ready for any downstream application.

  

Exosomes elute first and are followed by non-EV components
High-resolution fractionation protocol operated by gravity

SEC Fractionation

Human serum fractionation profile using Exo-spin™ SEC column. The protein concentration was estimated by Bradford assay (grey) and the particle concentration (blue) was measured by NTA.

 

What is the best Exo-spin™ product for my research? Contact us at tech@cellgs.com! And check the following papers to look into different concentration methods, if necessary.

  • Sheller-Miller S, Radnaa E, Arita Y, Getahun D, Jones RJ, Peltier M, Menon R. (2019). Environmental pollutant induced cellular injury is reflected in exosomes from placental explants. Placenta.
  • Kojima R, Bojar D, Rizzi G, Hamri GC, El-Baba MD, Saxena P, Ausländer S, Tan KR, Fussenegger M. (2018). Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment. Nature Communications 3;9(1):1305.
  • Shin La Shu, Yunchen Yang, Cheryl L. Allen, Edward Hurley, Kaity H. Tung, Hans Minderman, Yun Wu, Marc S. Ernstoff. (2019). Purity and yield of melanoma exosomes are dependent on isolation method. Journal of Extracellular Vesicles 9(1):1692401.
  • Bliss CM, Parsons AJ, Nachbagauer R, Hamilton JR, Cappuccini F, Ulaszewska M, Webber JP, Clayton A, Hill AVS, Coughlan L. (2019). Targeting Antigen to the Surface of EVs Improves the In Vivo Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice. Mol Ther Methods Clin Dev 16:108-125.

 

Learn about our exosome isolation range >> here.